{ "cells": [ { "cell_type": "markdown", "id": "e7d6d3ea", "metadata": {}, "source": [ "# scNT-seq: human hematopoiesis dynamics\n", "\n", "This tutorial demonstrates comprehensive analysis of single-cell hematopoiesis dynamics using {mod}`dynamo` with scNT-seq metabolic labeling data. We show how to perform absolute total RNA velocity analyses with the metabolic labeling datasets reported in the dynamo Cell paper, reproducing the total RNA velocity analyses from figure 3B. The {mod}`dynamo` package includes processed hematopoiesis data accessible via {meth}`dynamo.sample_data.hematopoiesis`." ] }, { "cell_type": "code", "execution_count": 1, "id": "c15f78dd-0b6b-4aa5-9f2d-187ca30a84c9", "metadata": {}, "outputs": [ { "name": "stdout", "output_type": "stream", "text": [ "Using already downloaded Arial font from: /tmp/dynamo_arial.ttf\n", "Registered custom font as: Arial\n", "\n", "\n", " ███ ████████ \n", "█████ █████ █████ █████ ███ █████ \n", " ██████ ██████ ██████ ████████ ████ \n", " ___ ████ ███\n", " | \\ _ _ _ _ __ _ _ __ ___ ███\n", " | |) | || | ' \\/ _` | ' \\/ _ \\█████ ███ \n", " |___/ \\_, |_||_\\__,_|_|_|_\\___/█████ ████ \n", " |__/ ███ █████ \n", "Tutorial: https://dynamo-release.readthedocs.io/ \n", " █████ \n", "\n" ] }, { "data": { "text/html": [ "
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