{ "cells": [ { "cell_type": "markdown", "id": "e8b67d91", "metadata": {}, "source": [ "# Molecular mechnism: Drivers of Rapid Cell Fate Transitions\n", "\n", "One intriguing phenomenon observed in hematopoiesis is that commitment to and appearance of the Meg lineage occurs more rapidly than other lineages (Sanjuan-Pla et al., 2013; Yamamoto et al., 2013). However, the mechanisms underlying this process remain elusive. To mechanistically dissect this finding, we focused on all cell types derived from the MEP-like lineage.\n", "\n", "In this tutorial, we will guide you to\n", "- learn vector field and manually select fixed points\n", "- visualize topography with computed fixed points\n", "- compute pseudotime (potential)\n", "- visualize vector field pseudotime of cell types\n" ] }, { "cell_type": "markdown", "id": "aca44e31", "metadata": {}, "source": [ "Import relevant packages" ] }, { "cell_type": "code", "execution_count": 1, "id": "51265933-8c0a-4915-91ef-ad5fe19ab679", "metadata": {}, "outputs": [ { "name": "stdout", "output_type": "stream", "text": [ "Using already downloaded Arial font from: /tmp/dynamo_arial.ttf\n", "Registered custom font as: Arial\n", "\n", "\n", " ███ ████████ \n", "█████ █████ █████ █████ ███ █████ \n", " ██████ ██████ ██████ ████████ ████ \n", " ___ ████ ███\n", " | \\ _ _ _ _ __ _ _ __ ___ ███\n", " | |) | || | ' \\/ _` | ' \\/ _ \\█████ ███ \n", " |___/ \\_, |_||_\\__,_|_|_|_\\___/█████ ████ \n", " |__/ ███ █████ \n", "Tutorial: https://dynamo-release.readthedocs.io/ \n", " █████ \n", "\n" ] }, { "data": { "text/html": [ "
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